Intracellular pH of stationary phase cells after 15 min in strong buffer

Value 6 Unitless Range: ±0.15 Unitless
Organism Budding yeast Saccharomyces cerevisiae
Reference Cimprich P, Slavík J, Kotyk A. Distribution of individual cytoplasmic pH values in a population of the yeast Saccharomyces cerevisiae. FEMS Microbiol Lett. 1995 Aug 1 130(2-3):245-51 p.248 right column 1st paragraph and fig.3bPubMed ID7649447
Method Fluorescence spectroscopy of an intracellular dye subjected to excitation at two different wavelengths.
Comments Fifteen minutes after resuspension in a strong buffer (0.2 M TEPA) the distribution was slightly narrower than stationary phase (BNID 105975)-full width at half maximum (of the Gaussian) is 0.36 instead of 0.4pH units. Range is standard deviation. n=60
Entered by Uri M
ID 105976