Duration of S phase (chromosome replication time)

Range ~55 Min
Organism Bacteria Escherichia coli
Reference Wang X, Liu X, Possoz C, Sherratt DJ. The two Escherichia coli chromosome arms locate to separate cell halves. Genes Dev. 2006 Jul 1 20(13):1727-31. p.1731 left column bottom paragraph (material and methods)PubMed ID16818605
Primary Source Wang X, Possoz C, Sherratt DJ. Dancing around the divisome: asymmetric chromosome segregation in Escherichia coli. Genes Dev. 2005 Oct 1 19(19):2367-77.PubMed ID16204186
Method P.1731 left column bottom paragraph: "Material and methods: E. coli AB1157 strains containing lacO and tetO arrays were constructed as in Lau et al. (2003). Conditions for bacterial growth and for visualizing fluorescent protein foci in living cells have been described in Wang et al. (primary source). During exponential growth in M9 minimal glycerol medium, cells had a τ of 100 min at 37°C, with nonoverlapping sequential G1 (B), S (C), and G2/M (D) phases, with S being ∼55 min and G2/M being ∼35 min (primary source). FISH was performed as in Bates and Kleckner (2005)."
Comments Note-value wasn't located in primary source
Entered by Uri M
ID 109249