Biosynthetic costs of deoxynucleotides

Range dATP 51.7~P: dCTP 48.7~P: dGTP 52.7~P: dTTP 47.7~P ~P (ATP)
Organism Unspecified
Reference Michael Lynch and Georgi K. Marinov, The bioenergetic costs of a gene, PNAS 2015 doi: 10.1073/pnas.1514974112 link Supplementary Materials p.1 right column top paragraph
Primary Source Akashi H, Gojobori T. Metabolic efficiency and amino acid composition in the proteomes of Escherichia coli and Bacillus subtilis. Proc Natl Acad Sci U S A. 2002 Mar 19 99(6):3695-700.PubMed ID11904428
Comments Supplementary Materials p.1 left column bottom paragraph:"Genome replication in free-living organisms requires the synthesis of new nucleotide triphosphates to create two new double helices from the parental DNA molecule. Such synthesis involves the use of PRPP (phosphoribosyl pyrophosphate) and amino acid molecules, which [investigators] assume must first also be synthesized, as well as additional investments of ATP in various steps in the purine and pyrimidine biosynthetic pathways. These pathways are generally conserved across the Tree of Life, and the total biosynthetic costs can be derived from the cumulative investments in each pathway step [supplementary refs 2, 3]. Using the costs (in ATP) for PRPP and individual amino acids outlined by Akashi and Gojobori [primary source] for a starting point of glucose, the biosynthetic costs are estimated to be: 51.7 P for dATP, 48.7 P for dCTP, 52.7 for dGTP, and 47.7 for dTTP. Similar estimates were presented by Wagner [supplementary ref 5]. Given the similarity of all four values, for the purposes herein, it will be sufficient to approximate the biosynthetic cost of each dNTP as 50 P."
Entered by Uri M
ID 112129