pH of resting mitochondria in living neonatal rat cardiomyocyte

Value 7.91 Unitless Range: ±0.16 Unitless
Organism Mammalian tissue culture cell
Reference Llopis J, McCaffery JM, Miyawaki A, Farquhar MG, Tsien RY. Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc Natl Acad Sci U S A. 1998 Jun 9 95(12):6803-8. p.6805 right column 2nd paragraph and fig.3dPubMed ID9618493
Method Compartment- Specific Expression of EYFPmito in Mammalian Cells. Fluorescence microscopy in living intact cell
Comments "The normal beating of cardiomyocytes expressing EYFP-mito suggests the noninvasive nature of these indicators." "The estimated resting pHm was 7.98±0.07 in HeLa cells (n=17 cells from six experiments) and 7.91±0.16 in cardiomyocytes (n=13 cells from six experiments). Similar pH values were obtained in a HeLa cell line stably expressing EYFP-mito." See BNID 105939
Entered by Uri M
ID 105940