pH of (probable) resting ER in living HeLa cell

Value 7.45 Unitless
Organism Human Homo sapiens
Reference Llopis J, McCaffery JM, Miyawaki A, Farquhar MG, Tsien RY. Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc Natl Acad Sci U S A. 1998 Jun 9 95(12):6803-8. p.6806 left column bottom line and p.6807 fig. 5bPubMed ID9618493
Method Compartment- Specific Expression of GT-EYFP in Mammalian Cells. Fluorescence microscopy in living intact cell
Comments "In cells expressing GT-EYFP, the protonophore CCCP (0.15mM) raised pHG to 7.1 (Fig. 5b), even in the presence of active H+ pumping. Bafilomycin A1 added next alkalinized the Golgi only slightly. In the experiment of Fig. 5b, one cell in the field was chosen as negative control because it showed a diffuse fluorescence staining (five times dimmer than that of Golgi area in neighboring cells). This staining likely corresponds to endoplasmic reticulum, because all GT-EYFP is synthesized there before export to the Golgi. This compartment had a resting pH of about 7.45 and, in contrast with pHG, it acidified slightly on CCCP addition (Fig. 5b)."
Entered by Uri M
ID 105942