Diffusion coefficient of HIV-1 particle in nucleus of human 293T cells

Range 50-220 nm^2/sec
Organism HIV
Reference Arhel N, Genovesio A, Kim KA, Miko S, Perret E, Olivo-Marin JC, Shorte S, Charneau P. Quantitative four-dimensional tracking of cytoplasmic and nuclear HIV-1 complexes. Nat Methods. 2006 Oct3(10):817-24PubMed ID16990814
Method Researchers labeled human immunodeficiency virus-1 (HIV-1) integrase with a small tetracysteine tag, which preserved the virus' infectivity while allowing it to be labeled with the bis-arsenical fluorescein derivative FlAsH. This labeling allowed them to image both intracytoplasmic and intranuclear HIV-1 complexes in three dimensions over time (4D) in human cells and enabled them to analyze HIV-1 kinetics by automated 4D quantitative particle tracking.
Comments After compensating for cell movements, researchers found that intranuclear complexes showed very slow and diffuse movements (movement IV), with an average speed of 0.003±0.0003 µm/sec (47 measurements). They found the diffusion coefficient (D) to be between 5e-5 - 2.2e-4 µm^2/sec for diffuse intranuclear movements (after fit with ?r^2=2nD?t, where n is the dimension 3).
Entered by Uri M
ID 104714