Half life of transcription factor mRNA in human cells

Value 2 Hours
Organism Human Homo sapiens
Reference Bernhard O. Palsson, Systems Biology: Properties of Reconstructed Networks, Cambridge University Press, pp. 12
Primary Source Decay Rates of Human mRNAs: Correlation With Functional Characteristics and Sequence Attributes, Genome Res. Yang et al. 13:1863-1872, 2003PubMed ID12902380
Method (Primary source): Researchers measured mRNA decay rates in two human cell lines (Hep G2-Hepatocellular carcinoma) and Bud8 primary cells with high-density oligonucleotide arrays that enable the measurement of decay rates simultaneously for thousands of mRNA species. To study the rates of mRNA degradation (“decay”) in human cells, they measured changes in mRNA levels following application of the RNA polymerase inhibitor Actinomycin D with Affymetrix U95Av2 high-density oligonucleotide arrays. They collected RNA from cells after 2–3 h of inhibition and used the Affymetrix Microarray Suite (MAS) 5.0 to analyze the changes from the untreated state. Four experiments (i.e., eight hybridizations) were performed in HepG2 cells, and they conducted an additional experiment in Bud8 primary cells to exclude the possibility of cancer-cell-specific artifacts.
Comments Combining the decay rate for all the probe sets present in the initial and final conditions, they find that the median half life in both cell types is ~10h. However, 5% of transcripts showed fast decay rates >0.5h^-1 or half life<2h, among which were the transcription factor transcripts. Biosynthesis and other housekeeping transcripts showed slower decay rates.
Entered by Ron Milo - Admin
ID 101091