Cytosolic pH during exponential growth on glucose

Value 7.2 pH units
Organism Budding yeast Saccharomyces cerevisiae
Reference Orij, Rick, et al. "In vivo measurement of cytosolic and mitochondrial pH using a pH-sensitive GFP derivative in Saccharomyces cerevisiae reveals a relation between intracellular pH and growth." Microbiology 155.1 (2009): 268-278. doi: 10.1099/mic.0.022038-0. abstract, p.274 right column bottom paragraph & p.275 right column bottom paragraphPubMed ID19118367
Method Measured using cytosolically targeted pH-sensitive GFP.
Comments "The in vivo measurement allowed accurate determination of organelle-specific pH, determining a constant pHcyt of 7.2 and a constant pHmit of 7.5 in cells exponentially growing on glucose." "[Researchers] determined a pHcyt of 7.2±0.2 and a pHmit of 7.5±0.2 in cells growing on glucose. These specific pH values appear to be tightly regulated and maintained in a broad range of external pH values, ranging from pH 3.0 to 7.5 (Figs 2c, 5a–c and 6). Previously reported pHi values were lower (Bracey et al., 1998 Fernandes et al., 2003 Guldfeldt & Arneborg, 1998). This is most likely due to the methods used, which determine an average pH over whole cells including the vacuole. This will result in significantly lower values."
Entered by Avi Flamholz
ID 109863