| Range |
35-3000 h-mtTFA/mtDNA
|
| Organism |
Human Homo sapiens |
| Reference |
Shutt TE, Lodeiro MF, Cotney J, Cameron CE, Shadel GS. Core human mitochondrial transcription apparatus is a regulated two-component system in vitro. Proc Natl Acad Sci U S A. 2010 Jul 6 107 (27):12133-8 p.12137 left column 1st paragraphPubMed ID20562347
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| Primary Source |
1)Cotney et al., (2007) Relative abundance of the human mitochondrial transcription system and distinct roles for h-mtTFB1 and h-mtTFB2 in mitochondrial biogenesis and gene expression. Nucleic Acids Res 35: 4042–4054. (2) Maniura-Weber et al., Transient overexpression of mitochondrial transcription factor A (TFAM) is sufficient to stimulate mitochondrial DNA transcription, but not sufficient to increase mtDNA copy number in cultured cells. Nucleic Acids Res. 2004 Nov 16 32(20):6015-27 (3) Takamatsu et al., Regulation of mitochondrial D-loops by transcription factor A and single-stranded DNA-binding protein. EMBO Rep. 2002 May3(5):451-6.PubMed ID17557812, 15547250, 11964388
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| Comments |
It is noteworthy that there are conflicting reports on relative abundance of h-mtTFA and mtDNA in vivo, ranging from 35 (primary source 2) to 50 (primary source 1) to approximately 3,000 h-mtTFA (note-primary source 3 gives value of 1,700 rather than 3,000) molecules per mtDNA molecule. The organizational units of mtDNA in vivo are nucleoids (27), which are protein–DNA complexes that contain 2–10 mtDNA molecules (28) that can be visualized with fluorescence microscopy as puncta in the organelle matrix. (numbers in parentheses point to references in article). |
| Entered by |
Uri M |
| ID |
105613 |