Intracellular asparagine concentration in glucose-fed, exponentially growing E. coli

Value 510 μM Range: Table - link μM
Organism Bacteria Escherichia coli
Reference Bennett BD, Kimball EH, Gao M, Osterhout R, Van Dien SJ, Rabinowitz JD. Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli. Nat Chem Biol. 2009 Aug5(8):593-9PubMed ID19561621
Method Researchers quantified metabolites by Liquid chromatography-mass spectrometry (LC-MS/MS) using an isotope ratio-based approach (8). As isotope-labeled standards for many metabolites are not available, they used uniformly 13C-labeled glucose medium to label the intracellular metabolome of E. coli (25). This enabled the use of commercially available unlabeled compounds as internal standards.
Comments For concentration in glycerol and acetate media see BNID 104704
Entered by Ron Milo, Paul Jorgensen, Mike Springer
ID 100159