Intracellular metabolite concentrations in glucose, glycerol, and acetate-fed, exponentially growing E. coli

Range Table - link
Organism Bacteria Escherichia coli
Reference Bennett BD, Kimball EH, Gao M, Osterhout R, Van Dien SJ, Rabinowitz JD. Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli. Nat Chem Biol. 2009 Aug5(8):593-9 Supplementary information pp.7-10 Supplementary table 3PubMed ID19561621
Method "[Investigators] quantified metabolites by LC-MS/MS [Liquid chromatography-mass spectrometry] using an isotope ratio-based approach (ref 8). As isotope-labeled standards for many metabolites are not available, [they] used uniformly 13C-labeled glucose medium to label the intracellular metabolome of E. coli (ref 25). This enabled the use of commercially available unlabeled compounds as internal standards."
Comments "TMFA [Thermodynamic metabolic flux analysis] was performed largely as originally described [ref 14]. Measured metabolite concentrations (Supplementary Table 3) were used to constrain the TMFA model." The total observed intracellular metabolite pool was approximately 300 mM.
Entered by Uri M
ID 104704