Molecular mass of voltage-gated potassium channel Shaker

Range ~400 kDa
Organism Fruit fly Drosophila melanogaster
Reference O. Sokolova, L.K. Partensky and N. Grigorieff, Three-Dimensional Structure of a Voltage-Gated Potassium Channel at 2.5 nm Resolution, Structure, Vol. 9, pages 215–220 (2001). p.216 left column top paragraphPubMed ID11286888
Method Abstract:"[Investigators] determined the three-dimensional structure of the entire Shaker channel at 2.5 nm resolution using electron microscopy."
Comments p.216 left column top paragraph:"The contour level chosen to generate the structure in Figure 3a is based on an average protein density of 810 Da/nm^3 [ref 15] and a volume giving roughly 400 kDa total mass, taking into account the ?6–46 deletion (see Experimental Procedures) and including glycosylation (approximately 16 kDa per subunit [ref 16]) and bound detergent (about 15 kDa per subunit, estimated by sedimentation [data not shown])." p.216 left column bottom paragraph:"The structure has a square shape and clearly shows two domains linked by thin connectors about 2 nm long. Based on their volume, the two domains have masses of about 300 kDa and 100 kDa, respectively."
Entered by Uri M
ID 111665