Free cytosolic phosphate concentration

Range 10 - 75 mM
Organism Budding yeast Saccharomyces cerevisiae
Reference van Eunen K et al., Measuring enzyme activities under standardized in vivo-like conditions for systems biology. FEBS J. 2010 Feb277(3):749-60. p.751 left column top paragraphPubMed ID20067525
Primary Source [14] Wu L et al., Short-term metabolome dynamics and carbon, electron, and ATP balances in chemostat-grown Saccharomyces cerevisiae CEN.PK 113-7D following a glucose pulse. Appl Environ Microbiol. 2006 May72(5):3566-77. [18] Auesukaree C, Homma T, Tochio H, Shirakawa M, Kaneko Y, Harashima S. Intracellular phosphate serves as a signal for the regulation of the PHO pathway in Saccharomyces cerevisiae. J Biol Chem. 2004 Apr 23 279(17):17289-94. [19] Gonzalez B, de Graaf A, Renaud M, Sahm H. Dynamic in vivo (31)P nuclear magnetic resonance study of Saccharomyces cerevisiae in glucose-limited chemostat culture during the aerobic-anaerobic shift. Yeast. 2000 Apr16(6):483-97. [20] Greenfield NJ, Hussain M, Lenard J. Effects of growth state and amines on cytoplasmic and vacuolar pH, phosphate and polyphosphate levels in Saccharomyces cerevisiae: a 31P-nuclear magnetic resonance study. Biochim Biophys Acta. 1987 Dec 7 926(3):205-14 [21] Theobald U, Mohns J & Rizzi M (1996) Determination of in-vivo cytoplasmic orthophosphate concentration in yeast. Biotechnol Tech 10, 297–302. link PubMed ID16672504, 14966138, 10790685, 3318934
Comments "From the elemental analysis, [investigators] could only estimate the total concentration of phosphorus, which was ~300 mM. A substantial part of this is present in bound phosphate groups or in the form of polyphosphates. To estimate the free cytosolic phosphate concentration, [investigators] used values from the literature. A broad range was found, from 10 to 75 mM [primary sources]. As the growth conditions applied by Wu et al. [primary source 14] were almost identical to [their] growth conditions, [they] used their value of 50 mM. However, [they] note that varying the phosphate concentration between 10 and 75 mM did not affect the reported Vmax values (Fig. S1), as reported below."
Entered by Uri M
ID 107757