Total amino acid concentration in cell (see comments section for total/free amino acid concentration)

Range ~150 mM
Organism Budding yeast Saccharomyces cerevisiae
Reference van Eunen K et al., Measuring enzyme activities under standardized in vivo-like conditions for systems biology. FEBS J. 2010 Feb277(3):749-60. p.752 right column bottom paragraphPubMed ID20067525
Primary Source [47] Canelas AB, Ras C, ten Pierick A, van Dam JC, Heijnen JJ & Van Gulik WM (2008) Leakage-free rapid quenching technique for yeast metabolomics. Metabolomics 4, 226–239. [52] Hans MA, Heinzle E, Wittmann C. Free intracellular amino acid pools during autonomous oscillations in Saccharomyces cerevisiae. Biotechnol Bioeng. 2003 Apr 20 82(2):143-51.PubMed ID12584756
Comments P.752 right column bottom paragraph:"An additional reason for this choice is that the total amino acid concentration in the cell is ∼ 150 mm [primary sources], which compensates substantially, albeit not completely, for the lack of anions." Please note-ref states "total amino acid" whereas primary source [52] gives "free intracellular amino acid".
Entered by Uri M
ID 107764