Rate of error in amino acylation of tRNA by aa-tRNA synthetase

Range 0.0001-0.00001 incorrect/correct amino acid
Organism Bacteria Escherichia coli
Reference Hani S. Zaher and Rachel Green, Fidelity at the Molecular Level: Lessons from Protein Synthesis. Cell Volume 136, Issue 4, 20 February 2009, Pages 746-762PubMed ID19239893
Primary Source C.S. Francklyn, DNA polymerases and aminoacyl-tRNA synthetases: shared mechanisms for ensuring the fidelity of gene expression, Biochemistry 47 (2008), pp. 11695–11703.PubMed ID18850722
Comments The aminoacylation step, carried out by the aa-tRNA synthetases (aaRSs), has been demonstrated to proceed with a remarkable level of accuracy the incorrect amino acid is attached to the tRNA once in 10^4-10^5 events, owing to kinetic discrimination and “double-sieve” editing mechanisms utilized by the enzymes. Note: Kramer et al 2007 PUBMEDID 17095544 give aminoacylation error rate of 10^-6 incorrect/correct aa
Entered by Uri M
ID 103469