Maximal emission wavelength for Venus fluorophore

Value 528 nm
Organism Aequorea victoria
Reference Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol. 2002 Jan20(1):87-90 p. 88 table 1 Table - link PubMed ID11753368
Method Random mutagenesis and gene construction: Random mutations were introduced in the gene for circularly permuted YFP (H148D/ V163A/S175G/Y203F)(Nagai et al. 2001 PMID 11248055) using the error-prone PCR method (Cadwell et al. 1994, PMID 7920233). Fluorescence of the bacterial colonies grown at 37°C on a plate was analyzed using researchers' homemade fluorescence image-analyzing system (Sawano et al. 2000, PMID 10931937).
Entered by Cellina Cohen-Saidon
ID 101100