| Comments |
P.3 2nd paragraph: "The precursors for most intrinsic chromophore FPs [fluorescent proteins] were isolated from marine organisms such as jellyfish, coral, and sea anemone (Chudakov, Matz, Lukyanov, & Lukyanov, 2010). The first engineered FPs were derived from GFP, with mutations to improve photostability, brightness,
folding, and monomericity, as well as chromophore mutations that altered
FP color (Chudakov et al., 2010 Cormack, Valdivia, & Falkow, 1996
Heim, Cubitt, & Tsien, 1995, Zacharias, Violin, Newton, & Tsien, 2002). Since then, several lineages of intrinsic chromophore FPs have emerged and been actively pursued, leading to a wide variety of proteins with different properties (Fig. 1, Table 1)." P.4 top paragraph: "Table 1 lists common intrinsic chromophore FPs, many of which of been incorporated into sensors." See notes beneath table |