Published values of protein and RNA contents

Range Table - link
Organism Bacteria Escherichia coli
Reference Taymaz-Nikerel H, Borujeni AE, Verheijen PJ, Heijnen JJ, van Gulik WM. Genome-derived minimal metabolic models for Escherichia coli MG1655 with estimated in vivo respiratory ATP stoichiometry. Biotechnol Bioeng. 2010 Oct 1 107(2):369-81. doi: 10.1002/bit.22802. p.375 table VPubMed ID20506321
Primary Source Emmerling M et al., Metabolic flux responses to pyruvate kinase knockout in Escherichia coli. J Bacteriol. 2002 Jan184(1):152-64. & Hua Q, Yang C, Baba T, Mori H, Shimizu K. Responses of the central metabolism in Escherichia coli to phosphoglucose isomerase and glucose-6-phosphate dehydrogenase knockouts. J Bacteriol. 2003 Dec185(24):7053-67. & Fischer E, Sauer U. A novel metabolic cycle catalyzes glucose oxidation and anaplerosis in hungry Escherichia coli. J Biol Chem. 2003 Nov 21 278(47):46446-51.PubMed ID11741855, 14645264, 12963713
Method Abstract:"The unknown ATP stoichiometry parameters of the constructed E. coli network were estimated from experimental data of eight different aerobic chemostat experiments carried out with E. coli MG1655, grown at different dilution rates (0.025, 0.05, 0.1, and 0.3 h(-1)) and on different carbon substrates (glucose, glycerol, and acetate)."
Comments "The macromolecular composition of the biomass is known to change with environmental conditions and specific growth rate (Bremer and Dennis, 1996). However, [investigators’] measurements of the protein content of E. coli cells grown in glucose limited chemostats showed that the protein content did not change significantly with changes in specific growth rate. The measured protein content agreed well with published data (Table V). Unfortunately the performed RNA measurements did not yield reproducible results: therefore published data (Table V), yielding a linear relation between RNA content and specific growth rate (Emmerling et al., 2002), were used to calculate the RNA content as a function of the specific growth rate."
Entered by Uri M
ID 111462