catalytic rate of rhodopsin in vitro

Value 46 sec^-1
Organism Generic
Reference Ernst OP, Gramse V, Kolbe M, Hofmann KP, Heck M. Monomeric G protein-coupled receptor rhodopsin in solution activates its G protein transducin at the diffusion limit. Proc Natl Acad Sci U S A. 2007 Jun 26104(26):10859-64. fig 5PubMed ID17578920
Method Construction of R–mVenus, R–mECFP, and mVenus–mECFP Fusion Plasmids, Rhodopsin, R–mVenus, R–mECFP, and mVenus–mECFP fusion proteins were expressed in COS-1 cells by using a diethylamino-ethyl (DEAE)-dextran transfection procedure, purification of rhodopsin, Preparation of Bovine Rhodopsin and SEC.
Comments In the native membrane catalytic rates are an order of magnitude higher. This may be explained by the reduction in translational degrees of freedom in a two-dimensional membrane, which facilitates successful coupling
Entered by Uri M
ID 103609