Estimated intracellular metabolite concentrations in saponin-isolated malaria parasites

Range Table - link mM
Organism Plasmodium falciparum
Reference Teng R, Junankar P, Bubb W, Rae C, Mercier P, et al. (2009) Metabolite profiling of the intraerythrocytic malaria parasite Plasmodium falciparum by 1H NMR spectroscopy. NMR Biomed 22: 292-302 DOI:10.1002/nbm.1323 pp.296-7 table 1PubMed ID19021153
Method "[Researchers] used 1H NMR spectroscopy to analyse the metabolome of mature, trophozoite-stage P. falciparum parasites, ‘isolated’ from the contents of their host erythrocytes by saponin-permeabilisation of the host erythrocyte membrane. Although it is recognised that the process of isolating parasites from their host cell will invariably lead to some loss of metabolites from the parasite (and hence to an underestimate of the intracellular concentrations of some solutes), the aim here was to identify parasite-specific compounds, as distinct from host cell metabolites."
Comments "Table 1 shows the estimated intracellular concentrations of 41 compounds or, in the case of the pyrimidine nucleotides, combinations of compounds, detected by 1H NMR in the different extracts of isolated P. falciparum trophozoites. For a number of other metabolites detected (e.g. pyruvate, citrate, hypoxanthine, IMP, NADP+, glycerophosphocholine and glycerophosphoethanolamine), the NMR peaks overlapped and/or were not sufficiently distinct to permit quantification." See notes beneath table
Entered by Uri M
ID 110870