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|Maass S et al.,Efficient, global-scale quantification of absolute protein amounts by integration of targeted mass spectrometry and two-dimensional gel-based proteomics. Anal Chem. 2011 Apr 1 83(7):2677-84. courtesy of authorPubMed ID21395229
|Easy, large-scale determination
of absolute protein concentrations was achieved by integrating
targeted mass spectrometric analyses of selected anchor
proteins with quantification of all proteins accessible on calibrated
two-dimensional (2-D) gels (PAGE-Polyacrylamide gel electrophoresis).
|B. subtilis grown in minimal medium and S. aureus grown in synthetic medium see supporting material p.S3