Estimate for percent of total energy production used for membrane energization in anaerobic conditions

Value 51 %
Organism Bacteria Escherichia coli
Reference Stouthamer, A. H. The Search for Correlation Between Theoretical and Experimental Growth Yields. 1979. from International Review of Biochemistry. Microbial Biochemistry, Vol. 21. ed. J. R. Quayle. 1979 University Park Press
Primary Source Stouthamer and Bettenhausen. A continuous culture study of an ATPase-negative mutant of Escherichia coli. Arch Microbiol. 1977. 113(3):185-9.PubMed ID141918
Comments In the ATPase negative mutant, respiration can energize the membrane, but the energetic potential cannot be used to create ATP. The ATPase negative mutant requires 57 mmol of ATP to produce 1g biomass. The wild-type E. coli grown anaerobically requires 117mmol ATP to create 1 g biomass. The difference between the two (61 mmol ATP) is the amount used to energize the membrane, assuming that the efficiency of biomass formation is the same in both conditions. See Szenk et al., 2017 PMID 28755958 p.103 left column 2nd paragraph: "First, it has been estimated that E. coli spends up to half of its energy maintaining ion gradients across a leaky inner membrane (primary source Stouthamer and Bettenhaussen, 1977), with this leakiness plausibly increasing as S/V (surface-to-volume ratio)."
Entered by Phil Mongiovi
ID 101779