| Comments |
In the ATPase negative mutant, respiration can energize the membrane, but the energetic potential cannot be used to create ATP. The ATPase negative mutant requires 57 mmol of ATP to produce 1g biomass. The wild-type E. coli grown anaerobically requires 117mmol ATP to create 1 g biomass. The difference between the two (61 mmol ATP) is the amount used to energize the membrane, assuming that the efficiency of biomass formation is the same in both conditions. See Szenk et al., 2017 PMID 28755958 p.103 left column 2nd paragraph: "First, it has been estimated that E. coli spends up to half of its energy maintaining ion gradients across a leaky inner membrane (primary source Stouthamer and Bettenhaussen, 1977), with this leakiness plausibly increasing as S/V (surface-to-volume ratio)." |