Cross section of polyhedral structures similar in size and appearance to carboxysomes

Range 100 - 200 nm
Organism Bacteria Salmonella enterica
Reference Bobik TA et al., The propanediol utilization (pdu) operon of Salmonella enterica serovar Typhimurium LT2 includes genes necessary for formation of polyhedral organelles involved in coenzyme B(12)-dependent 1, 2-propanediol degradation. J Bacteriol. 1999 Oct181(19):5967-75 p.5971 left column bottom paragraphPubMed ID10498708
Method EM, Electron Microscopy. P.5968 right column 3rd paragraph: "EM: Two fixation protocols were used. In the standard protocol, cells were fixed in 2% glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.2) for 30 min at room temperature and then in 1% osmium tetroxide in the same buffer for 1 h at 4°C."
Comments P.5971 left column bottom paragraph: "Formation of polyhedral bodies by S. enterica: S. enterica formed polyhedral structures similar in size and appearance to carboxysomes during aerobic growth on minimal 1,2-propanediol–B12 medium (Fig.1A), but not during aerobic growth on glucose (Fig. 1C). Polyhedra were 100 to 200 nm in cross-section and appeared to consist of a proteinaceous shell and interior. The bodies formed by S. enterica are somewhat irregular in shape compared to the carboxysomes of Thiobacillus neapolitanus, suggesting some differences between these structures (Fig. 1A and B)."
Entered by Uri M
ID 115210