Estimated fraction of membrane area occupied by glucose transporters when overflow metabolism begins

Value 1 %
Organism Bacteria Escherichia coli
Reference Szenk M, Dill KA, de Graff AMR. Why Do Fast-Growing Bacteria Enter Overflow Metabolism? Testing the Membrane Real Estate Hypothesis. Cell Syst. 2017 Aug 235(2):95-104. doi: 10.1016/j.cels.2017.06.005. p.96 right column bottom paragraphPubMed ID28755958
Comments P.96 right column bottom paragraph: "The second step of glucose uptake is through inner membrane transporters. [Investigators] estimate that glucose transporters occupy only 1% of the membrane area when overflow metabolism begins, and thus make a much smaller contribution to inner membrane crowding than the electron transport chain (Figure 2A and Box 1). This number is obtained using the area of a glucose transporter dimer (48 ± 10 nm^2) (Jeckelmann et al., 2011), its maximum uptake rate (kT=180 ± 40 glucose/s), the surface-to-volume ratio of a cell (Figure 1A), and the cell’s total glucose uptake rate (Vemuri et al., 2006, O’Brien et al., 2013) (Table S1A)."
Entered by Uri M
ID 114684