Diameter of MEFs (mouse embryonic fibroblasts)

Range 10 - 20 µm
Organism Mouse Mus musculus
Reference Turgay Y et al., (2017). The molecular architecture of lamins in somatic cells. Nature. 2017 Mar 9 543(7644):261-264. doi: 10.1038/nature21382 p.262 left column bottom paragraphPubMed ID28241138
Method Abstract: "Here [investigators] use cryo-electron tomography to obtain a detailed view of the organization of the lamin meshwork within the lamina. Data analysis of individual lamin filaments resolves a globular-decorated fibre appearance and shows that A- and B-type lamins assemble into tetrameric filaments of 3.5nm thickness."
Comments P.262 left column bottom paragraph: "Owing to their large size (~10–20 μm in diameter), nuclei could be easily identified at low magnification in cryo-EM images, confirming successful sample preparation and preservation of the overall size and shape of nuclei (Extended Data Fig. 2a–c)."
Entered by Uri M
ID 114286