Pyocyanin concentration in various media (see Method section for media content)

Range LB 3.42: MH 2.77: BHI 0.26: TS 0.23 μg/ml
Organism Bacteria Pseudomonas aeruginosa
Reference Iiyama K et al., Alkaline protease contributes to pyocyanin production in Pseudomonas aeruginosa. FEMS Microbiol Lett. 2017 Apr 1 364(7). doi: 10.1093/femsle/fnx051 p.3 right column top paragraphPubMed ID28333255
Method Abstract: "The role of the alkaline protease (AprA) in pyocyanin production in Pseudomonas aeruginosa was investigated. AprA was overproduced when a plasmid carrying the aprA gene was introduced to an aprA-deletion mutant strain, EG03, thus, aprA-complemented EG03 was used as an overproducing strain." P.2 left column 4th paragraph: "Luria-Bertani (LB, Lennox, Sigma-Aldrich, St. Louis, MO), brain–heart infusion (BHI, Nissui Pharmaceutical Co., Tokyo, Japan), BBL Mueller-Hinton (MH, Becton, Dickinson and Company, Franklin Lakes, NJ) and Bacto tryptic soy (TS, Becton, Dickinson and Company) broths were used as commercially available media."
Comments P.3 right column top paragraph: "Pyocyanin concentration was found to be varied among different media tested (Fig. 2). PAO1 produced pyocyanin at concentrations of 3.42, 2.77, 0.26 and 0.23 μg/ml in LB, MH, BHI and TS, respectively. In EG03, a significant decrease of pyocyanin concentration relative to that of PAO1 was observed only in LB. In LB and MH, the complemented strain and PAO1 produced comparable levels of pyocyanin. In BHI and TS, aprA complementation resulted in a remarkable increase in pyocyanin production, with concentrations reaching 14.0- and 3.6-fold in BHI and TS, respectively, relative to those observed with PAO1."
Entered by Uri M
ID 114112