Destabilizing effect of "average" mutation

Range ~1.3 (~5.5 kJ/mol) kcal/mol
Organism Generic
Reference Tokuriki N, Stricher F, Serrano L, Tawfik DS. How protein stability and new functions trade off. PLoS Comput Biol. 2008 Feb 29 4(2):e1000002. doi: 10.1371/journal.pcbi.1000002. p.1 abstractPubMed ID18463696
Method "[Researchers] performed a comprehensive analysis of the type, location, and stability effects of mutations that have conferred new enzymatic functions in laboratory evolution experiments."
Comments "[Researchers] found that mutations that modulate enzymatic functions are mostly destabilizing (average ??G = +0.9 kcal/mol), and are almost as destabilizing as the "average" mutation in these enzymes (+1.3 kcal/mol)."
Entered by Uri M
ID 109549