Hill coefficient for folate induced current and [H]+ concentration

Value 0.6 Unitless
Organism Mouse Mus musculus
Reference Umapathy NS, Gnana-Prakasam JP, Martin PM, Mysona B, Dun Y, Smith SB, Ganapathy V, Prasad PD. Cloning and functional characterization of the proton-coupled electrogenic folate transporter and analysis of its expression in retinal cell types. Invest Ophthalmol Vis Sci. 2007 Nov48(11):5299-305 Fig. 4PubMed ID17962486
Method Cloning of Mouse PCFT/HCP1 cDNA, Functional Expression of Mouse PCFT/HCP1 in X. laevis Oocytes,
Comments Hill coefficient is less than 1: Negatively cooperative reaction: Once one ligand molecule is bound to the enzyme, its affinity for other ligand molecules decreases. The data suggest that the transport process involves the influx of one H+ per transport cycle. Folate is a water-soluble vitamin, the cofactors of which are essential for the metabolism of one-carbon groups in biochemical reactions.
Entered by Uri M
ID 102597