| Value |
583
nm
|
| Organism |
Discosoma striata |
| Reference |
Patterson G, Day RN, Piston D. Fluorescent protein spectra, J Cell Sci. 2001 Mar114(Pt 5):837-8. p.838 table 1 link - link PubMed ID11181166
|
| Primary Source |
Matz MV, Fradkov AF, Labas YA, Savitsky AP, Zaraisky AG, Markelov ML, Lukyanov SA. Fluorescent proteins from nonbioluminescent Anthozoa species. Nat Biotechnol. 1999 Oct17(10):969-73. table 2PubMed ID10504696
|
| Method |
(Primary source) Proteins were expressed in E. coli with His6 tags at their N termini and purified using TALON metal affinity resin (Clontech). Proteins were at least 95% pure according to SDS–PAGE. Quantum yields for novel proteins were determined relative to wild-type GFP (Clontech). Perkin-Elmer LS50B spectrometer (Beaconsfield, UK) was used for quantitative measurements. All samples were excited at 470 nm, absorbance at this wavelength was 0.02, and excitation and emission slits were 5 nm. The spectra were corrected for photomultiplier response and monochromator transmittance, transformed to wave number and integrated. |
| Comments |
Protein originally from Discosoma striata, several rounds of optimziations via mutations were performed. The recent cloning of a gene that encodes a red fluorescent protein (dsRed) from the Indo-Pacific sea anemone Discosoma striata has provided yet another fluorescent protein that is further red-shifted (primary source). |
| Entered by |
Cellina Cohen-Saidon |
| ID |
101095 |