Dry weight of E. coli B/r cell in exponential phase on glucose medium, mass doubling time of 40 min

Value 280 fg Range: Table - link fg
Organism Bacteria Escherichia coli
Reference Neidhardt F.C. Escherichia coli and Salmonella: Cellular and Molecular Biology. Vol 1., Chapter 3 Chemical Composition of Escherichia coli Frederick C. Neidhardt and H. Edwin Umbarger ASM Press 1996. pp. 14 See comment C beneath table 1
Primary Source Additional ref - Physiology of the Bacterial Cell. Neidhardt F. C., Ingraham J.L., Schaechter M. Sinauer Associates Inc. 1990.
Method "Based on measurements of the total dry mass and the number of cells measured in portions of a reference culture (unpublished observations). The wet weight is calculated from the assumption that 70% of E. coli protoplasm is water. The total dry weight per cell is 2.8?10^-13g the water content (assuming that 70% of the cell is water[PMID 1960728]) is 6.7?10^-13g the total weight of one cell is 9.5?10^-13g."
Comments Table gives general information on quantities of macromolecules per cell in balanced growth at 37°C in glucose minimal medium. Mass doubling time: 40min. Cell size is dependent on growth rate. The dry weight of one cell must be scaled appropriately for different growth rates. weight/cell is given in Atkinson, B., and Mavituna, F. 1983. Biochemical Engineering and Biotechnology Handbook, The Nature Press, N.Y., U.S.A. For cell dry mass of 422 fg on unknown media (same reference) see BNID 103892
Entered by Uri M
ID 103904