GFP diffusion rate in water

Value 87 µm^2/sec
Organism Generic
Reference Swaminathan, R., C. P. Hoang, and A. S. Verkman. Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion, Biophys J. 1997 Apr72(4):1900-7. p.1903 left column top paragraphPubMed ID9083693
Method p.1901 left column top paragraph:"Studies using recombinant purified GFP in solution and GFP transfected in cell cytoplasm indicated that GFP-S65T has suitable photochemical properties for cellular photobleaching and polarization measurements. Spot photobleaching was then used to quantify GFP translational diffusion in cell cytoplasm, and time-resolved anisotropy was used to quantify GFP rotational diffusion."
Comments "The deduced GFPS65T diffusion coefficient from the t1/2 value was 8.67×10^-7cm2/s, in agreement with that of 8.7×10-7cm2/s for wild-type GFP determined by fluorescence correlation microscopy (Terry et al., 1995 PMID 8526912)."
Entered by Ron Milo - Admin
ID 100301