|Swaminathan, R., C. P. Hoang, and A. S. Verkman. Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion, Biophys J. 1997 Apr72(4):1900-7. p.1903 left column top paragraphPubMed ID9083693
|p.1901 left column top paragraph:"Studies using recombinant
purified GFP in solution and GFP transfected in cell cytoplasm
indicated that GFP-S65T has suitable photochemical
properties for cellular photobleaching and polarization measurements.
Spot photobleaching was then used to quantify
GFP translational diffusion in cell cytoplasm, and time-resolved
anisotropy was used to quantify GFP rotational
|"The deduced GFPS65T
diffusion coefficient from the t1/2 value was 8.67×10^-7cm2/s, in agreement with that of 8.7×10-7cm2/s for
wild-type GFP determined by fluorescence correlation microscopy
(Terry et al., 1995 PMID 8526912)."
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