Mean lifetime of transient dendritic filopodia on immature dendrites

Value 9.5 min Range: ±7.7 min
Organism Rat Rattus norvegicus
Reference Ziv NE, Smith SJ. Evidence for a role of dendritic filopodia in synaptogenesis and spine formation. Neuron. 1996 Jul17(1):91-102. P.93 right column bottom paragraphPubMed ID8755481
Method Abstract:"Axo-dendritic synaptogenesis was examined in live hippocampal cell cultures using the fluorescent dyes DiO to label dendrites and FM 4-64 to label functional presynaptic boutons."
Comments P.93 right column bottom paragraph: "Transient dendritic filopodia on immature dendrites (9–11 DIV [days in vitro]) had a mean lifetime of 9.5 ± 7.7 min (mean and standard deviation, 63 filopodia, five experiments, same neurons as in Figure 4B). For this analysis, [investigators] necessarily considered only filopodia that both appeared and disappeared during the time-lapse session, while filopodia that were discernible either at the beginning or at the end of the time-lapse session (about 33%) were excluded. This selection procedure may discriminate against the more persistent filopodia, and [investigators’] mean lifetime values may therefore underestimate those that would be obtained with longer sampling periods."
Entered by Uri M
ID 112039