Estimated protein synthesis rate

Value 1.8 mg protein/gram FW/day
Organism Thale cress Arabidopsis thaliana
Reference Piques et al., Ribosome and transcript copy numbers, polysome occupancy and enzyme dynamics in Arabidopsis. Mol Syst Biol. 2009 5: 314 p.4 left column top paragraphPubMed ID19888209
Method Two complementary approaches were taken to quantify cytosolic and plastid ribosomes: The first approach used qRT–PCR. The second approach used relative quantitative proteomics based on emPAI values (Ishihama et al, 2005) to estimate the abundance of ribosomal proteins in the polysome gradient fractions.
Entered by Uri M
ID 107723