Ribosomal occupancy (of 98 transcripts analyzed)

Range 40 to 95% in the dark period: 50 to 90% in the light period %
Organism Thale cress Arabidopsis thaliana
Reference Piques et al., Ribosome and transcript copy numbers, polysome occupancy and enzyme dynamics in Arabidopsis. Mol Syst Biol. 2009 5: 314 p.4 right column 2nd paragraph and fig.3PubMed ID19888209
Method Two complementary approaches were taken to quantify cytosolic and plastid ribosomes: The first approach used qRT–PCR. The second approach used relative quantitative proteomics based on emPAI values (Ishihama et al, 2005) to estimate the abundance of ribosomal proteins in the polysome gradient fractions.
Comments The proportion of a transcript present in polysomes is often termed ‘ribosome occupancy'.
Entered by Uri M
ID 107726