Number of different aptamer oligonucleotide sequences in first round of SELEX

Range 10^15 Unitless
Organism Unspecified
Reference Rimmele M. Nucleic acid aptamers as tools and drugs: recent developments. Chembiochem. 2003 Oct 6 4(10):963-71.PubMed ID14523912
Method Systematic Evolution of Ligands by EXponential enrichment (SELEX). SELEX comprises an iterative process of in vitro selections of nucleic-acid target-binding events and partitioning events of unbound nucleic acids and is used to isolate high-affinity nucleic acid ligands from large pools of randomized nucleic acid sequence libraries.
Comments Nucleic acid aptamers are molecules that bind to their ligands with high affinity and specificity. Unlike other functional nucleic acids such as antisense oligonucleotides, ribozymes, or siRNAs, aptamers almost never exert their effects on the genetic level. Note-value of 10^14-10^15 given by Win and Smolke in Chapter 8.3.3 of 'The metabolic pathway engineering handbook' (CRC Press), ed. C.D. Smolke 2010.
Entered by Uri M
ID 105439