Burst size of S3 myophage

Value 100 Virions/cell
Organism Bacteriophage S3 (host Stenotrophomonas)
Reference García P. et al., Isolation of new Stenotrophomonas bacteriophages and genomic characterization of temperate phage S1. Appl Environ Microbiol. 2008 Dec74 (24):7552-60 abstract & p.7554 right column 5th paragraphPubMed ID18952876
Method p.7553 left column 4th paragraph:"Host range, phage purification, and functional studies: Phage host ranges were determined by placing 5-µl drops of each viral suspension onto lawns of each of the 26 Stenotrophomonas strains. Large-scale phage propagation and purification through CsCl gradients were performed as described in reference 30 [Suárez, J. E., and K. F. Chater. 1981. Development of a DNA cloning system in Streptomyces using phage vectors. Cienc. Biol. 6:99-110.]. Curves from one-step analyses of growth in 2x TY at a multiplicity of infection of 0.03 were generated as described in reference 15 [Herrero, M., C. G. de los Reyes-Gavilán, J. L. Caso, and J. E. Suárez. 1994. Characterization of f393-A2, a bacteriophage that infects Lactobacillus casei. Microbiology 140:2585-2590.]."
Comments "The lytic cycles lasted 30 min for S3 and about 75 min for S1 and S4. The burst size for S3 was 100 virions/cell, while S1 and S4 produced about 75 virus particles/cell."
Entered by Uri M
ID 104852