Number of neomycin resistance gene (NEO) mRNAs per cell

Value 230 mRNA/cell Range: ±31 mRNA/cell
Organism Mouse Mus musculus
Reference Fujimoto J, Narayanan CS, Benjamin JE, Heinflink M, Gershengorn MC. Mechanism of regulation of thyrotropin-releasing hormone receptor messenger ribonucleic acid in stably transfected rat pituitary cells. Endocrinology. 1992 Apr130(4):1879-84PubMed ID1312428
Method Researchers study the mechanism of regulation of TRH-R mRNA in a line of GH3 cells that are stably transfected with mouse pituitary TRH-R cDNA (GH-mTRHR-1 cells).
Comments GH-mTRHR-1 cells contained 26±1.6 molecules of mouse TRH-R mRNA/cell and 230±31 molecules of mRNA for the neomycin resistance gene (NEO) with which it was cotransfected.
Entered by Uri M
ID 104206