Decrease in Coenzyme Q mitochondrial concentration that does not affect electron transport

Value <= % Range: 60-70 %
Organism Nematode Caenorhabditis elegans
Reference Turunen M, Olsson J, Dallner G. Metabolism and function of coenzyme Q. Biochim Biophys Acta. 2004 Jan 28 1660(1-2):171-99.PubMed ID14757233
Primary Source Asencio C, Rodríguez-Aguilera JC, Ruiz-Ferrer M, Vela J, Navas P. Silencing of ubiquinone biosynthesis genes extends life span in Caenorhabditis elegans.FASEB J. 2003 Jun17(9):1135-7.PubMed ID12709403
Method By RNA interference (RNAi) eight genes, including clk-1, involved in ubiquinone biosynthesis. in C. elegans feeding animals with dsRNA-containing Escherichia coli HT115 strains. Gene interference. HPLC
Comments The analysis of Q9 content by HPLC showed that it was diminished by 60% in N2 strain after 2 weeks silencing clk-1/coq7 (Fig. 2, Fig. 3 upper panel), and absent in the negative control clk-1 qm51 mutant strain. However, no significant accumulation of demethoxy-Q9 (DMQ9) was detected in coq-7/clk-1-interfered as verified for clk-1 mutants
Entered by Uri M
ID 103287