Subcellular concentrations of adenine nucleotides in perfused livers from fed and starved rats

Range Table - link
Organism Rat Rattus norvegicus
Reference Soboll S, Scholz R, Heldt HW. Subcellular metabolite concentrations. Dependence of mitochondrial and cytosolic ATP systems on the metabolic state of perfused rat liver. Eur J Biochem. 1978 Jun 15 87(2):377-90. p.384 table 5PubMed ID668699
Method “From marker enzyme activities and metabolite contents in the unfractionated homogenate and in the different fractions the mitochondrial and cytosolic metabolite contents (nmol/mg protein) were calculated by extrapolating the data to fractions of pure mitochondria and pure cytosol according to Elbers et al. [ref 5]. The subcellular metabolite contents were converted into concentrations assuming a water content of 0.8 and 3.8 µl/mg protein in the mitochondrial and cytosolic compartment, respectively, based on considerations previously discussed by Soboll et al. [ref 1].”
Comments "Changes in the mitochondrial ATP/ADP ratios within the range of 0.1 and 0.8 were also found in perfused livers from fed and starved rats (Table 5). It is conceivable that the regulation of pyruvate metabolism in perfused liver is exerted by changes of mitochondrial ATP and ADP concentrations. [Researchers’] data are consistent with the regulatory requirements in the two different metabolic states: pyruvate oxidation in the fed state requires an active pyruvate dehydrogenase and low ATP/ADP ratios (see Table 5, expt F: 0.16), whereas gluconeogenesis in the starved state requires an activation of pyruvate carboxylase and an inactivation of pyruvate dehydrogenase favoured by high ATP/ADP ratios (see Table 5, expts H, H-D, H-L: 0.6-0.7)." For experimental conditions and metabolic states see p.381 table 1 link
Entered by Uri M
ID 111357