Mean half-life of total labeled RNA

Range ~5 days
Organism Human Homo sapiens
Reference Defoiche J et al., Measurement of ribosomal RNA turnover in vivo by use of deuterium-labeled glucose. Clin Chem. 2009 Oct55(10):1824-33. p.1830 left column 2nd paragraphPubMed ID19696118
Method For method development in vitro, a lymphocyte cell line (PM1) was cultured in the presence of [6,6-2H2]-glucose. RNA was extracted, hydrolyzed enzymatically to ribonucleosides, and derivatized to either the aldonitrile tetra-acetate or the pentafluoro triacetate derivative of the pentose before GC-MS.
Comments Labeled total RNA was lost at a rate (l*) of about 15%/day, equivalent to a mean half-life (t1/2) of about 5 days.
Entered by Uri M
ID 108026