Cell surface area (average±standard deviation) of outer membrane (OM) and plasma membrane (PM) obtained from serial ultrathin sectioning

Range OM 4.31±0.75: PM 3.76±0.69 μm^2
Organism Bacteria Escherichia coli
Reference Hiroyuki Yamada et al., Structome analysis of Escherichia coli cells by serial ultrathin sectioning reveals the precise cell profiles and the ribosome density, Microscopy, Volume 66, Issue 4, August 2017, Pages 283–294, link p.288 table 3 PubMed ID28854579
Method P.286 left column bottom paragraph: "In this study, E. coli was selected as a target of the structome analysis because a large number of molecular biological studies were performed with this microorganism. E. coli cells were cut into 65 nm thick serial ultrathin sections after rapid cryofixation and freeze-substitution. In addition to the structome analysis by serial ultrathin sectioning, PCM (phase contrast microscopy) and SEM (scanning electron microscopy) examinations were performed on the same bacterial sample because the fundamental morphological studies were achieved with these observation techniques."
Comments P.288 left column, 2nd paragraph: "Two-dimensional profiles for the nine E. coli cells were calculated from values measured using ImageJ/Fiji software, as described in the Materials and methods section. Table 3 lists the surface areas of the cell (OM) and plasma membrane (PM) in nine cells. The average cell (OM) and PM surface areas were 4.31 ± 0.75 μm^2 (ranging from 3.35 to 5.61 μm^2) and 3.76 ± 0.69 μm^2 (ranging from 2.85 to 4.76 μm^2), respectively."
Entered by Ron Milo - Admin
ID 117346