Cellular fluorescence signal to target for detection of bacterial contamination (emission peak)

Value 340 nm
Organism bacteria
Reference Dartnell LR, Roberts TA, Moore G, Ward JM, Muller JP. Fluorescence characterization of clinically-important bacteria. PLoS One. 2013 Sep 308(9):e75270. doi: 10.1371/journal.pone.0075270. eCollection 2013. p.10 left column, bottom paragraphPubMed ID24098687
Method Abstract: "[Investigators] have characterized the complete fluorescence response of eleven clinically-relevant bacteria by generating excitation-emission matrices (EEMs) over broad wavelength ranges."
Comments P.10 left column, bottom paragraph: "All the examined bacterial strains exhibited a distinctive double-peak fluorescence feature of tryptophan, a protein-bound aromatic amino acid, but no other cellular fluorophore was detected. Thus, this fluorescence survey found that an emission peak of 340 nm is the cellular fluorescence signal to target for detection of bacterial contamination, maximally excited with a UV illumination source of 280 nm."
Entered by Ron Milo - Admin
ID 117340