| Range |
≤300 - 500 g/L
|
| Organism |
Unspecified |
| Reference |
Theillet FX et al., Physicochemical properties of cells and their effects on intrinsically disordered proteins (IDPs). Chem Rev. 2014 Jul 9 114(13):6661-714. doi: 10.1021/cr400695p p.6685 right column 2nd paragraphPubMed ID24901537
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| Primary Source |
[114] Zimmerman SB, Trach SO. Estimation of macromolecule concentrations and excluded volume effects for the cytoplasm of Escherichia coli. J Mol Biol. 1991 Dec 5 222(3):599-620 [648] Zimmerman SB, Minton AP. Macromolecular crowding: biochemical, biophysical, and physiological consequences. Annu Rev Biophys Biomol Struct. 1993 22: 27-65 DOI: 10.1146/annurev.bb.22.060193.000331PubMed ID1748995, 7688609
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| Method |
Primary source [114] abstract: "The very high concentration of macromolecules within cells can potentially have an overwhelming effect on the thermodynamic activity of cellular components because of excluded volume effects. To estimate the magnitudes of such effects, [investigators] have made an experimental study of the cytoplasm of Escherichia coli. Parameters from cells and cell extracts are used to calculate approximate activity coefficients for cytoplasmic conditions. These calculations require a representation of the sizes, concentrations and effective specific volumes of the macromolecules in the extracts. Macromolecule size representations are obtained either by applying a two-phase distribution assay to define a related homogeneous solution or by using the molecular mass distribution of macromolecules from gel filtration. Macromolecule concentrations in cytoplasm are obtained from analyses of extracts by applying a correction for the dilution that occurs during extraction. That factor is determined from experiments based upon the known impermeability of the cytoplasmic volume to sucrose in intact E. coli." |
| Comments |
P.6685 right column 2nd paragraph: "As outlined in section 2.1, intracellular environments and extracellular fluids contain large amounts of macromolecules, reaching concentrations of up to 300–500 g/L, including proteins, nucleic acids, lipids, glycans, metabolites, and solvated ions (primary sources)." Primary source [114] abstract: "Macromolecule concentrations in the cytoplasm of E. coli in either exponential or stationary growth phase are estimated to be approximately 0.3 to 0.4 g/ml." Please note-primary source [114] studied E. coli |
| Entered by |
Uri M |
| ID |
116348 |