Translational diffusion of GFP in cell

Range 3-8 µm^2/sec
Organism Bacteria Escherichia coli
Reference Theillet FX et al., Physicochemical properties of cells and their effects on intrinsically disordered proteins (IDPs). Chem Rev. 2014 Jul 9 114(13):6661-714. doi: 10.1021/cr400695p p.6668 left column 3rd paragraphPubMed ID24901537
Primary Source [164] Mika JT, Poolman B. Macromolecule diffusion and confinement in prokaryotic cells. Curr Opin Biotechnol. 2011 Feb22(1):117-26. doi: 10.1016/j.copbio.2010.09.009PubMed ID20952181
Method Primary source abstract: "[Investigators] review recent observations on the mobility of macromolecules and their spatial organization in live bacterial cells. [They] outline the major fluorescence microscopy-based methods to determine the mobility and thus the diffusion coefficients (D) of molecules, which is not trivial in small cells."
Comments P.6668 left column 3rd paragraph: "Translational diffusion of GFP [∼27 kDa, hydrodynamic radius (RH) 2.82 nm] in E. coli is ∼10 times slower (Dcyt = 3–8 μm^2/s) than in water (D = 87 μm^2/s)(primary source)."
Entered by Uri M
ID 115862