| Value |
500
µm
|
| Organism |
Bacteria Salmonella typhimurium |
| Reference |
Young KD. The selective value of bacterial shape. Microbiol Mol Biol Rev. 2006 Sep70(3):660-703 DOI: 10.1128/MMBR.00001-06 p.685 right column 3rd paragraphPubMed ID16959965
|
| Primary Source |
[88] Gaze, W. H., N. Burroughs, M. P. Gallagher, and E. M. Wellington. 2003. Interactions between Salmonella typhimurium and Acanthamoeba polyphaga, and observation of a new mode of intracellular growth within contractile vacuoles. Microb. Ecol. 46: 358–369 DOI: 10.1007/s00248-003-1001-3PubMed ID14502413
|
| Method |
Primary source [88] abstract: "Acanthamoeba polyphaga feeding on Salmonella typhimurium in a simple model biofilm were observed by light microscopy and a detailed record of interactions kept by digital image capture and image analysis. A strain of S. typhimurium SL1344 carrying a fis: gfp reporter construct (pPDT105) was used to assess intracellular growth in A. polyphaga on non-nutrient agar (NNA) plates." |
| Comments |
P.685 right column 3rd paragraph: "An intriguing observation is that members of the commonly studied Enterobacteriaceae respond in a similar way to predation by protozoa. Acanthomoeba polyphaga ingests Salmonella enterica serovar Typhimurium, and, at a very low rate, the bacterium grows in the contractile vacuoles of the amoeba
(primary source). The intracellular bacteria grow as filaments, with cell lengths increasing from 2µm to 18µm and reaching upwards of 75 to 150 or 500µm after several days (primary source). The cells continue to grow as filaments even after being released from the amoeba, and they often form small microcolonies of filamentous cells (primary source). Analogous behavior is observed when E. coli is ingested (primary source). These reactions parallel those of urinary tract pathogenic strains of E. coli, which will be discussed at more length in “DIFFERENTIATION” below." |
| Entered by |
Uri M |
| ID |
115725 |