| Value |
6000
SipA molecules/expressing bacterium
Range: ±3,000 SipA molecules/expressing bacterium
|
| Organism |
Bacteria Salmonella typhimurium |
| Reference |
Schlumberger MC et al., Real-time imaging of type III secretion: Salmonella SipA injection into host cells. Proc Natl Acad Sci U S A. 2005 Aug 30 102(35):12548-53 DOI: 10.1073/pnas.0503407102 abstract, p.12549 right column 3rd paragraph & p.12551 left column bottom paragraphPubMed ID16107539
|
| Method |
Abstract: "By using time-lapse microscopy, [investigators] have imaged delivery of the Salmonella type III effector protein SipA/SspA into animal cells in real time. SipA delivery mostly began 10-90 sec after docking and proceeded for 100-600 sec until the bacterial SipA pool (6 +/- 3 x 10^(3) molecules) was exhausted." |
| Comments |
P.12549 right column 3rd paragraph: "SipA is highly expressed by ≈26% of the S. typhimurium population (6 ± 3 × 10^3 SipA molecules per expressing bacterium) (Fig. 1 A and B see also Materials and Methods, Fig. 5, which is published as supporting information on the PNAS web site, and Supporting Materials and Methods)." P.12551 left column bottom paragraph: "In conclusion, M1304 bacteria harbor ≈6 ± 3 × 10^3 SipA molecules before host cell contact (see Fig. 1 A and B ) and require ≈100-600 sec to deliver this cargo into the host cell, which roughly corresponds to translocation rates between 7 and 60 molecules per sec (see Supporting Materials and Methods)." |
| Entered by |
Uri M |
| ID |
115218 |