Time after contact with host animal cell that SipA (type III effector protein) pool delivery begins and delivery time

Range beginning of delivery (time after contact) 10-90 sec: delivery time of SipA pool≈100-600 sec
Organism Bacteria Salmonella typhimurium
Reference Schlumberger MC et al., Real-time imaging of type III secretion: Salmonella SipA injection into host cells. Proc Natl Acad Sci U S A. 2005 Aug 30 102(35):12548-53 DOI: 10.1073/pnas.0503407102 abstract & p.12551 left column bottom paragraphPubMed ID16107539
Method Abstract: "By using time-lapse microscopy, [investigators] have imaged delivery of the Salmonella type III effector protein SipA/SspA into animal cells in real time. SipA delivery mostly began 10-90 sec after docking and proceeded for 100-600 sec until the bacterial SipA pool (6 +/- 3 x 10^(3) molecules) was exhausted."
Comments P.12551 left column bottom paragraph: "In conclusion, M1304 bacteria harbor ≈6 ± 3 × 10^3 SipA molecules before host cell contact (see Fig. 1 A and B ) and require ≈100-600 sec to deliver this cargo into the host cell, which roughly corresponds to translocation rates between 7 and 60 molecules per sec (see Supporting Materials and Methods)."
Entered by Uri M
ID 115219