Lower limit for reliable detection of GFPs [Green Fluorescent Proteins]

Range ~1,400 molecules/cell
Organism Budding yeast Saccharomyces cerevisiae
Reference Ho B, Baryshnikova A, Brown GW. Unification of Protein Abundance Datasets Yields a Quantitative Saccharomyces cerevisiae Proteome. Cell Syst. 2018 Feb 28 6(2):192-205.e3. doi: 10.1016/j.cels.2017.12.004 p.202 right column 2nd paragraphPubMed ID29361465
Method P.202 right column 2nd paragraph: "In conclusion, [investigators] provide a comprehensive view of protein abundance in yeast by normalizing and combining 21 abundance datasets, collected by MS [Mass Spectrometry], GFP fluorescence flow cytometry, GFP fluorescence microscopy, and western blotting. Since cellular autofluorescence interferes with detection of GFP fluorescence, [they] find that the lower limit for reliable detection of GFP proteins corresponds to ∼1,400 molecules per cell. Above this threshold [they] found less variation among GFP-based studies than among MS studies, suggesting that although MS analyses provide the greatest sensitivity and dynamic range for protein measurements, the GFP-based measurements have greater precision."
Entered by Uri M
ID 115096