Protein abundance

Range range 0 - 750,000: median 2,622: 67% of proteins between 1,000 - 10,000 molecules/cell
Organism Budding yeast Saccharomyces cerevisiae
Reference Ho B, Baryshnikova A, Brown GW. Unification of Protein Abundance Datasets Yields a Quantitative Saccharomyces cerevisiae Proteome. Cell Syst. 2018 Feb 28 6(2):192-205.e3. doi: 10.1016/j.cels.2017.12.004 p.198 left columnPubMed ID29361465
Method Abstract: "[Investigators] evaluated 21 quantitative analyses of the S. cerevisiae proteome, normalizing and converting all measurements of protein abundance into the intuitive measurement of absolute molecules per cell."
Comments P.198 right column: "With [investigators’] unified dataset, [they] find that yeast protein abundance, when logarithmically transformed, is skewed toward high-abundance proteins (Figure 4C). Protein abundance ranges from zero to 7.5 × 10^5 molecules per cell, the median abundance is 2,622 molecules per cell, and 67% of proteins quantified exist between 1,000 and 10,000 molecules per cell (Figure 4C). Low-abundance proteins, the first decile, have abundances ranging from 3 to 822 molecules per cell, while high-abundance proteins, the tenth decile, have abundances ranging from 1.4 × 10^5 to 7.5 × 10^5. [Their] data suggest that protein copy number is maintained within a narrow range from which only a small portion of the proteome deviates." P.202 right column 2nd paragraph: "Collectively, [investigators’] analyses indicate that protein abundance in the yeast proteome ranges from 3 to 7.5 × 10^5 molecules per cell, with a median abundance of 2,622 molecules per cell. [They] define the lowest abundance proteins as those present at 866 or fewer copies, and the highest abundance proteins as those with 14,938 or more copies."
Entered by Uri M
ID 115090