| Method |
Abstract: "[Investigators] used whole-cell recordings from Im [main intercalated nucleus] neurons in acute slices of mouse amygdala... [They] also functionally studied Im cell inputs. Excitatory postsynaptic currents (eEPSCs) were evoked by the stimulation of the Im, intermediate capsula (IC), external capsula (EC) or BLA [basolateral complex of the amygdala], when GABAergic transmission was pharmacologically blocked. An occlusion test indicated that fibres recruited by stimulating Im and IC, or Im and EC were distinct. These eEPSCs had both NMDA [N-methyl-D-aspartate] and AMPA [α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid] receptor components. Inhibitory postsynaptic currents (eIPSCs) were evoked after the stimulation of the Im, the EC and the BLA, when glutamatergic transmission was pharmacologically blocked." |
| Comments |
P.1914 right column bottom paragraph: "The position of the Im [intercalated nucleus] was identified online with infrared and DIC [differential interference contrast] optics, and could be recognized as a cluster of densely packed cells located ventrally to the BLA [basolateral complex of the amygdala] nucleus (Fig. 1A). All slices used were prepared from bregma level ∼−0.9 mm (Paxinos & Franklin, 2007) (Fig. 1A, B and C). Post hoc anatomical confirmation of the location of the somata of the recorded neurons within the Im was performed in the great majority of the experiments (see Methods). Intercalated cell (ITC) clusters consist of at least two cell types, so‐called medium and large ITCs (Millhouse, 1986). The majority of ITCs consist of medium ITCs (∼95%) that have round to elongated somata and a round to bipolar dendritic tree bearing dendritic spines (Millhouse, 1986, Royer et al. 1999, 2000, Marowsky et al. 2005, Geracitano et al. 2007). [Investigators] first measured basic electrophysiological responses of Im cells (n= 45), (Fig. 1D, E and F, Table 1), and found that their passive membrane properties were typical of small neurons (e.g. Rin= 641 ± 20 MΩ Cm= 30 ± 2 pF)." |